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Registros recuperados: 22
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A 66-kDa protein of bovine hypophyseal Pars tuberalis induces luteinizing hormone release from rat Pars distalis Biocell
Lafarque,Martha; Oliveros,Liliana.
In this study, evidence for a factor secreted by bovine hypophyseal pars tuberalis that stimulates luteinizing hormone (LH) release from rat pars distalis cells is shown. The secretion products of bovine pars tuberalis cells into the culture medium were assayed on dispersed rat pars distalis cells in 30 min incubations and superfusion experiments. The culture medium from pars tuberalis total cell populations, added at a dose of 6 μg per tube, induced the greater LH release from pars distalis cells, without effect on follicle stimulating hormone (FSH) release. After pars tuberalis cells separation on a discontinuos Percoll gradient, only the culture medium of cells from 50 and 60% strength Percoll were able to release LH from rat pars distalis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adenohypophysis; Gonadotropes; Lactotrophs; Follicle stimulating hormone; Prolactin.
Ano: 2008 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000300001
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Antisense mRNA for NPY-Y1 receptor in the medial preoptic area increases prolactin secretion BJMBR
Silveira,N.A.; Franci,C.R..
We investigated the participation of neuropeptide Y-Y1 receptors within the medial preoptic area in luteinizing hormone, follicle-stimulating hormone and prolactin release. Four bilateral microinjections of sense (control) or antisense 18-base oligonucleotides of messenger ribonucleic acid (mRNA) (250 ng) corresponding to the NH2-terminus of the neuropeptide Y1 receptor were performed at 12-h intervals for two days into the medial preoptic area of ovariectomized Wistar rats (N = 16), weighing 180 to 200 g, treated with estrogen (50 µg) and progesterone (25 mg) two days before the experiments between 8.00 and 10:00 a.m. Blockade of Y1 receptor synthesis in the medial preoptic area by the antisense mRNA did not change plasma luteinizing hormone or...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neuropeptide Y; Medial preoptic area; Neuropeptide Y-Y1 receptor; Prolactin; MRNA for neuropeptide; Y-Y1 receptor; Luteinizing hormone; Follicle-stimulating hormone.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900016
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Candidate gene variation in gilthead sea bream reveals complex spatiotemporal selection patterns between marine and lagoon habitats ArchiMer
Guinand, B.; Chauvel, C.; Lechene, M.; Tournois, J.; Tsigenopoulos, C. S.; Darnaude, A. M.; Mckenzie, David; Gagnaire, P. A..
In marine fishes, the extent to which spatial patterns induced by selection remain stable across generations remains largely unknown. In the gilthead sea bream Sparus aurata, polymorphisms in the growth hormone (GH) and prolactin (Prl) genes can display high levels of differentiation between marine and lagoon habitats. These genotype-environment associations have been attributed to differential selection following larval settlement, but it remains unclear whether selective mortality during later juvenile stages further shapes genetic differences among habitats. We addressed this question by analysing differentiation patterns at GH and Prl markers together with a set of 21 putatively neutral microsatellite loci. We compared genetic variation of spring...
Tipo: Text Palavras-chave: Candidate gene; Growth hormone; Prolactin; Genetic differentiation; Amplicon sequencing; Local selection.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00625/73751/74235.pdf
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Decreased spermatogenic and androgenic testicular functions in adult rats submitted to immobilization-induced stress from prepuberty BJMBR
Almeida,S.A.; Petenusci,S.O.; Anselmo-Franci,J.A.; Rosa-e-Silva,A.A.M.; Lamano-Carvalho,T.L..
We investigated whether chronic stress applied from prepuberty to full sexual maturity interferes with spermatogenic and androgenic testicular functions. Male Wistar rats (40 days old) were immobilized 6 h a day for 60 days. Following immobilization, plasma concentrations of corticosterone and prolactin increased 135% and 48%, respectively, while plasma luteinizing hormone and testosterone presented a significant decrease of 29% and 37%, respectively. Plasma concentration of follicle-stimulating hormone was not altered in stressed rats. Chronic stress reduced the amount of mature spermatids in the testis by 16% and the spermatozoon concentration in the cauda epididymidis by 32%. A 17% reduction in weight and a 42% decrease in DNA content were observed in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Spermatogenesis; LH; FSH; Testosterone; Prolactin.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100013
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Dopaminergic modulation of grooming behavior in virgin and pregnant rats BJMBR
Serafim,A.P.; Felicio,L.F..
Dopamine receptors are involved in the expression of grooming behavior. The pregnancy-induced increase in self-licking observed in rats is important for mammary gland development and lactation. This study focuses on the role of dopamine receptor subtypes in grooming behavior of virgin and pregnant female rats. General and mammary gland grooming were measured in virgin rats treated with 0.25 mg/kg of the D1-like agonist SKF-81297 and antagonist SKF-83566 and the D2-like agonist lisuride and antagonist sulpiride. The effects of 0.01 and 0.25 mg/kg doses of the same agonists and antagonists were evaluated in pregnant rats as well. In virgin animals both SKF-83566 and sulpiride treatments significantly reduced the time spent in general grooming, while none of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Self-licking; Lisuride; SKF-81297; SKF-83566; Sulpiride; Mammary gland; Prolactin; Dopamine.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100015
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Effects on prolactin secretion and binding to dopaminergic receptors in sleep-deprived lupus-prone mice BJMBR
Palma,B.D.; Hipolide,D.C.; Tufik,S..
Sleep disturbances have far-reaching effects on the neuroendocrine and immune systems and may be linked to disease manifestation. Sleep deprivation can accelerate the onset of lupus in NZB/NZWF1 mice, an animal model of severe systemic lupus erythematosus. High prolactin (PRL) concentrations are involved in the pathogenesis of systemic lupus erythematosus in human beings, as well as in NZB/NZWF1 mice. We hypothesized that PRL could be involved in the earlier onset of the disease in sleep-deprived NZB/NZWF1 mice. We also investigated its binding to dopaminergic receptors, since PRL secretion is mainly controlled by dopamine. Female NZB/NZWF1 mice aged 9 weeks were deprived of sleep using the multiple platform method. Blood samples were taken for the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Systemic lupus erythematosus; NZB/NZWF1 mice; Prolactin; Stress; Autoradiography; Dopamine transporter.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300012
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Evaluación genético-molecular de pie de cría suizo americano en el estado de Chiapas. Colegio de Postgraduados
Alfonso Ruiz, Edy.
Un estudio genético molecular fue realizado en la región tropical de Chiapas, México, para conocer las características morfométricas y estimar el polimorfismo de los genes Prolactina y Kappa caseína en una población de ganado Suizo Americano. La extracción de ADN se obtuvo de muestras de sangre y mediante PCR-RFLP se determinaron el polimorfismo de prolactina (PRL) y kappa caseína (K-CSN). En los animales en estudio se obtuvieron medidas morfométricas, clasificándolos en grupos dentro de la raza. Usando el PROC GLM de SAS se realizaron análisis exploratorios multivariados de Componentes Principales para definir los morfotipos y mediante el uso del software POPGENE se estimaron distancias e identidades genéticas, así como las frecuencias génicas y...
Palavras-chave: Prolactina; K-CSN; Morfometría; Suizo Americano; Prolactin; Morphometrics; American Swiss; Doctorado; Ganadería.
Ano: 2011 URL: http://hdl.handle.net/10521/502
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Evaluación genético-molecular de pie de cría suizo americano en el estado de Chiapas. Colegio de Postgraduados
Alfonso Ruiz, Edy.
Un estudio genético molecular fue realizado en la región tropical de Chiapas, México, para conocer las características morfométricas y estimar el polimorfismo de los genes Prolactina y Kappa caseína en una población de ganado Suizo Americano. La extracción de ADN se obtuvo de muestras de sangre y mediante PCR-RFLP se determinaron el polimorfismo de prolactina (PRL) y kappa caseína (K-CSN). En los animales en estudio se obtuvieron medidas morfométricas, clasificándolos en grupos dentro de la raza. Usando el PROC GLM de SAS se realizaron análisis exploratorios multivariados de Componentes Principales para definir los morfotipos y mediante el uso del software POPGENE se estimaron distancias e identidades genéticas, así como las frecuencias génicas y...
Palavras-chave: Prolactina; K-CSN; Morfometría; Suizo Americano; Prolactin; Morphometrics; American Swiss; Doctorado; Ganadería.
Ano: 2011 URL: http://hdl.handle.net/10521/502
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Evidence of a role for prolactin as regulators of ovarian follicular development in goose Electron. J. Biotechnol.
Ma,Rong; Jiang,Dongmei; Chen,Ziyu; Kang,Bo.
Background Prolactin (PRL) regulates development and reproduction, and its effects are mediated by the prolactin receptor (PRLR). In order to clarify the role of PRLR and PRL in the process of follicular development in the goose ovary, the level of PRLR mRNA expression in the ovary and follicles of the Sichuan white goose was determined, as well as the PRL concentration in ovarian follicles. Results The level of PRLR mRNA in the hierarchical follicles (HFs) initially increased, and subsequently decreased, whereas PRLR expression was initially low and later increased in postovulatory follicles (POFs). The level of PRLR mRNA expression was the highest in the F4 follicles, and lowest in the F1 follicles in all of the examined follicles. Compared with the...
Tipo: Journal article Palavras-chave: Postovulatory follicle; Preovulatory follicle; Prolactin receptor; Prolactin; Sichuan white goose.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600001
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Histo-cytological responses of the prolactin cells of the catfish Heteropneustes fossilis to cadmium exposure Acta Toxicol. Argent.
Srivastav,A.K; Rai,Rubi; Mishra,D; Srivastav,S. K; Suzuki,Nobuo.
Cadmium is an important metal for modern industrial processes and, being biologically non-essential, poses health hazards to the organisms. In this study we aimed to evaluate the effect of cadmium exposure on the histo-cytology of prolactin cells in the freshwater catfish, Heteropneustes (H.) fossilis. Fish were subjected to 288 mg/L (0.8 of 96 h LC50) and 72 mg/L (0.2 of 96 h LC50) of cadmium chloride for short-term and long-term, respectively. After sacrificing the fish, the blood was collected on 24, 48, 72 and 96 h in short-term and after 7, 14, 21, and 28 days in long-term experiment and analyzed for plasma calcium levels. Also, pituitary glands were fixed on these intervals. The plasma calcium levels of short-term cadmium exposed fish remain...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cadmium; Fish; Prolactin; Pituitary gland.
Ano: 2014 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-37432014000300002
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Increased expression of SNARE proteins and synaptotagmin IV in islets from pregnant rats and in vitro prolactin-treated neonatal islets Biol. Res.
CUNHA,DANIEL A; AMARAL,MARIA EC; CARVALHO,CAROLINA PF; COLLARES-BUZATO,CARLA B; CARNEIRO,EVERARDO M; BOSCHERO,ANTONIO C.
During pregnancy and the perinatal period of life, prolactin (PRL) and other lactogenic substances induce adaptation and maturation of the stimulus-secretion coupling system in pancreatic β-cells. Since the SNARE molecules, SNAP-25, syntaxin 1, VAMP-2, and synaptotagmins participate in insulin secretion, we investigated whether the improved secretory response to glucose during these periods involves alteration in the expression of these proteins. mRNA was extracted from neonatal rat islets cultured for 5 days in the presence of PRL and from pregnant rats (17th-18th days of pregnancy) and reverse transcribed. The expression of genes was analyzed by semi-quantitative RT-PCR assay. The expression of proteins was analyzed by Western blotting and...
Tipo: Journal article Palavras-chave: Insulin; Pancreatic islets; Pregnant rats; Prolactin; SNARE proteins; Synaptotagmin.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300016
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Investigation of PRL-RsaI and HaeIII gene polymorphisms in Anatolian water buffaloes bred by using PCR-RFLP method R. Bras. Zootec.
Özşensoy,Yusuf.
ABSTRACT The objective of this study was to investigate polymorphisms both in exons 1 and 3 of prolactin (PRL) gene for milk productivity of Anatolian water buffalo breed in Sivas province in Turkey. Blood samples were collected from 129 male and female water buffaloes and DNA was isolated by using phenol/chloroform method. Samples of DNA were amplified and resulting PCR products were digested with RsaI (for exons 1 and 3) and HaeIII (for exon 1). Allelic polymorphisms were determined by separation of fragments obtained from digested PCR products in 3% agarose gel electrophoresis. AA genotype (HaeIII) and BB genotype (RsaI) of exon 1 and only AA genotype (RsaI) of exon 3 were obtained. No polymorphisms were determined in Anatolian water buffalo breed and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HaeIII; PCR-RFLP; Prolactin; RsaI; Sivas.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100403
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Is the infertility in hypothyroidism mainly due to ovarian or pituitary functional changes? BJMBR
Armada-Dias,L.; Carvalho,J.J.; Breitenbach,M.M.D.; Franci,C.R.; Moura,E.G..
The objective of the present study was to examine whether hypothyroidism affects the reproductive system of adult female rats by evaluating ovarian morphology, uterus weight and the changes in serum and pituitary concentrations of prolactin and gonadotropins. Three-month-old female rats were divided into three groups: control (N = 10), hypothyroid (N = 10), treated with 0.05% 6-propyl-2-thiouracil (PTU) in drinking water for 60 days, and T4-treated group (N = 10), receiving daily sc injections of L-thyroxine (0.8 µg/100 g body weight) during the last 10 days of the experiment. At the end of 50 days of hypothyroidism no hypothyroid animal showed a regular cycle, while 71% of controls as well as the T4-treated rats showed regular cycles. Corpora lutea,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hypothyroidism; Prolactin; Gonadotropins; Folliculogenesis; Estrous cycle.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900015
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Methylmercury inhibits prolactin release in a cell line of pituitary origin BJMBR
Maués,L.A.L.; Macchi,B.M.; Crespo-López,M.E.; Nasciutti,L.E.; Picanço-Diniz,D.L.W.; Antunes-Rodrigues,J.; Nascimento,J.L.M. do.
Heavy metals, such as methylmercury, are key environmental pollutants that easily reach human beings by bioaccumulation through the food chain. Several reports have demonstrated that endocrine organs, and especially the pituitary gland, are potential targets for mercury accumulation; however, the effects on the regulation of hormonal release are unclear. It has been suggested that serum prolactin could represent a biomarker of heavy metal exposure. The aim of this study was to evaluate the effect of methylmercury on prolactin release and the role of the nitrergic system using prolactin secretory cells (the mammosomatotroph cell line, GH3B6). Exposure to methylmercury (0-100 μM) was cytotoxic in a time- and concentration-dependent manner, with an LC50...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mercury; Methylmercury; Prolactin; Oxidative stress; Pituitary; Nitric oxide.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800691
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Molecular characterisation of prolactin and analysis of extrapituitary expression in the European sea bass Dicentrarchus labrax under various salinity conditions ArchiMer
Boutet, Isabelle; Lorin Nebel, C; De Lorgeril, Julien; Guinand, B.
dAlthough prolactin has been demonstrated to be the main hormone involved in adaptation to dilute media in several freshwater teleosts, few studies have been conducted in marine teleosts. In the Mediterranean, the sea bass Dicentrarchus labrax inhabits environments ranging from the open sea to coastal lagoons, where salinity varies greatly. We characterised the prolactin (prl) gene and analysed its expression in two organs (gill and intestine) in D. labrax acclimated to either freshwater or seawater. A 2819 bp long sequence encompassing the prl gene and a part (282 bp) of the promoter were identified, and these comprised 5 coding exons separated by 4 introns. Prolactin was similarly expressed in fresh- and seawater adapted fish, although expression in...
Tipo: Text Palavras-chave: Prolactin; Phylogenctic analysis; MRNA expression; Osmoregulation; Intestine; Gill; Dicentrarchuslabrax.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2537.pdf
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Prolactin and cortisol levels in women with endometriosis BJMBR
Lima,A.P.; Moura,M.D.; Rosa e Silva,A.A.M..
Endometriosis is a progressive estrogen-dependent disease affecting women during their reproductive years. The objective of the present study was to investigate whether endometriosis is associated with stress parameters. We determined cortisol and prolactin levels in serum, peritoneal and follicular fluid from infertile women with endometriosis and fertile women without the disease. The extent of the disease was staged according to the revised American Fertility Society classification (1997). Serum and peritoneal fluid were collected from 49 women aged 19 to 39 years undergoing laparoscopy. Eighteen women had stage I-II endometriosis and 10 had stage III-IV. Controls were 21 women undergoing laparoscopy for tubal sterilization. Follicular fluid was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endometriosis; Stress; Infertility; Prolactin; Cortisol.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800015
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Pup age and aggressive behavior in lactating rats BJMBR
Giovenardi,M.; Consiglio,A.R.; Barros,H.M.T.; Lucion,A.B..
High levels of aggressive behaviors against intruders in the nest area are displayed by female rats during the first 10 days after delivery, declining thereafter to very low levels, even though lactation continues. Cross-fostering experiments were undertaken to test the hypothesis that pup age may affect aggression in lactating rats. The behavior of females on the 8th day after delivery when raising fostered 8-day-old pups was compared to that of females on the 8th postpartum day raising older pups (18 days old) for the last 5 days, and females on the 18th day after delivery raising fostered 18-day-old pups were compared to females in the same postpartum period nursing younger pups (8 days of age at the time of the maternal aggression test) for 5 days. Pup...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Maternal aggression; Mother-infant interaction; Cross-fostering; Prolactin.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900015
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Relative Concentrations of Placental Lactogen II and PRL-Like Protein-A in Stressed Rats Placenta International Journal of Morphology
Romanini,M. C; Paz,D. A.; Rodríguez,N; Rolando,A. N.; Mugnaini,M. T.; Soñez,C. A.; Bozzo,A; Marioli,J. M.; Pastorino,I. C; Gauna,H. F..
The chronic stress induces functional adaptations in the hypothalamo-pituitary- adrenocortical (HPA) and in the sympathetic-medullary-adrenal axis (SAM). Both axis are considered vital regulators of the homeostasis in vertebrates (Seyle, 1936; Ostrandrer et al, 2006. On the other hand, the placenta provides highly specialized functions during gestation that are critical for the normal development of the embryo/fetus (Soares et al., 1991). We hypothesized that the chronic immobilization (IMO) stress in pregnancy rats produces alterations in prolactin concentrations in placental tissue and also changes in the response of SAM axis. Chronic stress by IMO was applied on days 12, 17 and 21 of pregnancy rats. Relative concentrations and localization of placental...
Tipo: Journal article Palavras-chave: Chronic stress; Placental lactogen; Prolactin; Rat; SAM.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000100011
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Reproductive experience influences grooming behavior during pregnancy in rats BJMBR
Serafim,A.P.; Felicio,L.F..
The pregnancy-induced increase in self-licking observed in rats is important for mammary gland development and lactation. Reproductive experience has epidemiologial implications such as a decrease in the incidence of mammary gland cancer in women and it also influences various behavioral, neurochemical and endocrine parameters. The aim of the present study was to investigate the influence of reproductive experience on grooming behavior patterns during pregnancy in rats. Self-grooming behavior was measured in age-matched virgin, primi- and multigravid (days 7, 8, 9, 19, and 20 of pregnancy) rats. General grooming (head, forelimbs and shoulders) was not significantly different among virgin, primi- and multigravid rats during pregnancy. Confirming previous...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Self-licking; Parity; Lactation; Mammary gland; Prolactin; Dopamine.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300016
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Social condition affects hormone secretion and exploratory behavior in rats BJMBR
Genaro,G.; Schmidek,W.R.; Franci,C.R..
Studies of behavior, endocrinology and physiology have described experiments in which animals housed in groups or in isolation were normally tested individually. The isolation of the animal from its group for testing is perhaps the most common situation used today in experimental procedures, i.e., there is no consideration of the acute stress which occurs when the animal is submitted to a situation different from that it is normally accustomed to, i.e., group living. In the present study, we used 90 male 120-day-old rats (Rattus norvegicus) divided into 5 groups of 18 animals, which were housed 3 per cage, in a total of 6 cages. The animals were tested individually or with their groups for exploratory behavior. Hormones were determined by radioimmunoassay...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Isolation; Group; Stress; Reproduction; ACTH; Luteinizing hormone; Corticosterone; Prolactin.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600008
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